HEATR1 Negatively Regulates Akt to Help Sensitize Pancreatic Cancer Cells to Chemotherapy
PUBLISHED: 2016-02-23  758 total views, 1 today

Tongzheng Liu1Yuan Fang2Haoxing Zhang1,3Min Deng1Bowen Gao1Nifang Niu4Jia Yu3SeungBaek Lee1JungJin Kim1Bo Qin1Fang Xie4,Debra Evans1Liewei Wang4Wenhui Lou2,*, and Zhenkun Lou1,*

Author Affiliations

1Division of Oncology Research, Department of Oncology, Mayo Clinic, Rochester, Minnesota.
2Department of Pancreatic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
3School of Life Sciences, Southwest University, Chongqing, China.
4Division of Clinical Pharmacology, Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, Minnesota.

*Corresponding Authors:
Zhenkun Lou, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Phone: 507-284-2702; Fax: 507-293-0107; E-mail: lou.zhenkun@mayo.edu; and Wenhui Lou, Fudan University, 180 Fenglin Rd, Shanghai, 200032, China. E-mail: lou.wenhui@zs-hospital.sh.cn   T. Liu and Y. Fang contributed equally to this article.

Abstract

Elucidating mechanisms of chemoresistance is critical to improve cancer therapy, especially for the treatment of pancreatic ductal adenocarcinoma (PDAC). Genome-wide association studies have suggested the less studied gene HEAT repeat-containing protein 1 (HEATR1) as a possible determinant of cellular sensitivity to different chemotherapeutic drugs. In this study, we assessed this hypothesized link in PDAC, where HEATR1 expression is downregulated significantly. HEATR1 silencing in PDAC cells increased resistance to gemcitabine and other chemotherapeutics, where this effect was associated with increased AKT kinase phosphorylation at the Thr308 regulatory site. Mechanistically, HEATR1 enhanced cell responsiveness to gemcitabine by acting as a scaffold to facilitate interactions between AKT and the protein phosphatase PP2A, thereby promoting Thr308 dephosphorylation. Consistent with these findings, treatment with the AKT inhibitor triciribine sensitized HEATR1-depleted PDAC cells to gemcitabine, suggesting that this therapeutic combination may overcome gemcitabine resistance in patients with low HEATR1 expression. Clinically, we found that HEATR1 downregulation in PDAC patients was associated with increased AKT phosphorylation, poor response to tumor resection plus gemcitabine standard-of-care treatment, and shorter overall survival. Collectively, our findings establish HEATR1 as a novel regulator of AKT and a candidate predictive and prognostic indicator of drug responsiveness and outcome in PDAC patients.

The copyright reseverd by Cancer Res; 76(3); 572–81. ©2015 AACR. Please log on Cancer Res to view the full text. http://cancerres.aacrjournals.org/content/76/3/572.abstract

Author
Dr. Lou Wenhui
+ Author Profile

Dr. Lou Wenhui

MD, Professor of Surgery, CSCO membership


Graduated in Shanghai Medical University in 1992 as Bachelor of Medicine, he obtained his M.D degree in 1999.

 

From 2001, he focused his career in pancreatic neoplasm and neuroendocrine tumors, now he is the Director of Departmentof Pancreatic Surgery;Vice-Director, Department of General Surgery, Zhong Shan Hospital, Fudan University; meanwhile he is Designated Chair of Shanghai Surgical Association; Vice-Chair of Chinese Society of Neuroendocrine Tumor; Fellow of Chinese College of Surgery; Member of Steering Committee, Pancreatic Disease Committee, Chinese Medical Doctor Association.

 

In past ten years, he has published morethan 30 papers in the field of pancreatic tumor in international magazine; and been invited to give lectures in international symposium. He is also responsible for many national research project, including National Nature Science Fund, “863” Hi-Tech Research Project etc. 

Email: lou.wenhui@zs-hospital.sh.cn

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